Arabidopsis STAY-GREEN, Mendel's Green Cotyledon Gene, Encodes Magnesium-Dechelatase

Y Shimoda, H Ito, A Tanaka - The Plant Cell, 2016 - academic.oup.com
… germ protein expression system converted chlorophyll a to … Next, we examined the Mg-dechelating
activity of mature SGR … the resulting free chlorophylls. The other hypothesis is that …

Structural characterization of magnesium dechelatase and chlorophyllide a oxygenase involved in the chlorophyll metabolic pathway

D DEY - 2022 - eprints.lib.hokudai.ac.jp
… Both chlorophylls possess distinct absorption spectra in the blue and … which catalyzes Mg2+
dechelation from chlorophyll a. This … The Arabidopsis SGR-less mutants showed substantial …

Chlorophyll a is a favorable substrate for Chlamydomonas Mg-dechelatase encoded by STAY-GREEN

K Matsuda, Y Shimoda, A Tanaka, H Ito - Plant physiology and …, 2016 - Elsevier
… in Arabidopsis cells, the chlorophyll content decreased, further confirming that SGR has
Mg-dechelating activity in … Conversion of chlorophyll b to chlorophyll a is indispensable for …

[HTML][HTML] Crystal structure and catalytic mechanism of 7-hydroxymethyl chlorophyll a reductase

X Wang, L Liu - Journal of Biological Chemistry, 2016 - ASBMB
… -reaction in chlorophyll b to chlorophyll a conversion. HCAR is … Here, we report the crystal
structure of Arabidopsis HCAR at 2.7-… Chlorophylls were extracted with acetone from spinach …

Horizontal transfer of promiscuous activity from nonphotosynthetic bacteria contributed to evolution of chlorophyll degradation pathway

D Obata, A Takabayashi, R Tanaka… - … Biology and Evolution, 2019 - academic.oup.com
… Mg-dechelation activities against chlorophylls in PSI particles … the highest activity among
Arabidopsis SGRs. We propose that Mg-… The dechelation of Mg from chlorophyll b results in the …

Catalytic and structural properties of pheophytinase, the phytol esterase involved in chlorophyll breakdown

L Guyer, K Salinger, U Krügel… - Journal of experimental …, 2018 - academic.oup.com
… By testing different porphyrin substrates with recombinant pheophytinase from Arabidopsis
… intermediate of chlorophyll biosynthesis. Thus, preceding Mg-dechelation over dephytylation …

[HTML][HTML] HCAR Is a Limitation Factor for Chlorophyll Cycle and Chlorophyll b Degradation in Chlorophyll-b-Overproducing Plants

X Zhao, T Jia, X Hu - Biomolecules, 2020 - mdpi.com
… leaf senescence, because magnesium (Mg) cannot be extracted from Chl b by Mg-… /b ratio
seems to be determined by the Chl a to b conversion in the Chl cycle. Transgenic Arabidopsis, …

The biochemistry, physiology, and evolution of the chlorophyll cycle

A Tanaka, R Tanaka - Advances in Botanical Research, 2019 - Elsevier
… a chlorophyll b-less Arabidopsis mutant compliments its chlorophyll b deficiency, while
transformation … These observations suggest that the differential distribution of chlorophylls among …

[HTML][HTML] In vitro demetalation of central magnesium in various chlorophyll derivatives using Mg-dechelatase homolog from the chloroflexi Anaerolineae

S Sato, M Hirose, R Tanaka, H Ito, H Tamiaki - Photosynthesis Research, 2024 - Springer
… of chlorophylls (Chls), an enzyme called STAY-GREEN or SGR catalyzes the removal of the
central magnesium … and investigated their in vitro dechelations using an SGR enzyme. Chl-a …

[HTML][HTML] Insights into the structure and function of the rate-limiting enzyme of chlorophyll degradation through analysis of a bacterial Mg-dechelatase homolog

D Dey, D Dhar, H Fortunato, D Obata, A Tanaka… - Computational and …, 2021 - Elsevier
… ) gene catalyzes Mg 2+ dechelation from chlorophyll a. This … the extraction of magnesium
(Mg) ion from chlorophyll a to form … functional similarity with the Arabidopsis thaliana SGR-Like (…